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Whole genome sequencing and BGC analysis
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Genomic DNA from Paenibacillus sp. M2 was isolated using a DNA isolation kit (Promega) and sequenced using Nanopore and Illumina MiSeq. Unicycler was used to perform a hybrid assembly of Illumina and Oxford Nanopore reads35. SPAdes was first used to generate a short-read assembly graph, which was scaffolded with long reads. Multiple rounds of polishing were conducted using Pilon. A single contig was obtained and analyzed using antiSMASH 6.036 to identify the BGC responsible for LAR synthesis.
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