Injection of IMP in aneurysm models and formation of a meniscus

The aneurysm model was filled with fluid (either blood or water) including the aneurysm’s cavity and then IMP (either air or FC-40) was injected into the model, followed by an injection of the fluid to trap the IMP between fluid phases. The injection of the fluid and the IMP was done using syringes connected to syringe pumps as mentioned above. The amount of injected IMP depends on the geometry and volume of the model and parent artery, and it should completely cover the neck of the aneurysm. For the ideal and physiological models, 300 to 500 μl of IMP was injected. For blood experiments, whole blood was taken from healthy human volunteers and supplied by the Israeli National Blood Service [Rambam Medical Center Institutional Review Board (IRB) RMB-0413-21].