Definition of cold/hot herbs’ targets and network construction

BW Boyang Wang
LX Lihao Xiao
PC Pan Chen
TZ Tingyu Zhang
PZ Peng Zhang
LC Liang Cao
ZZ Ziyi Zhou
HC Haibo Cheng
TZ Tong Zhang
SL Shao Li
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Taking the complex composition of herbs into consideration, many biomolecular might be the potential targets for many herbs. A strategy was implemented there to define targets for Hot herbs, Cold herbs and both Cold/Hot herbs. For each target, the counts of Cold/Hot herbs targeting on it were normalized on the total amount of Cold/Hot herbs and the ratio of the normalized counts of Cold/Hot herbs was used to divided targets into three groups, including targets of Hot herbs, targets of Cold herbs and shared targets. For Cold/Hot ZHENG CG, the biological network was constructed by the seeds genes with VIP value larger than 1 and DEGs in both CSG and CAG. Node side of each seeds gene depended on the VIP value. Besides, the biological networks for the targets of Cold/Hot herbs were constructed by the unions of targets of Cold/Hot herbs, respectively and seeds genes with VIP value larger than 1. All the gene–gene interactions in these networks were collected from STRING database [73].

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