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Live/Dead Assay

YW Yumeng Wu
TY Tatsuya Yano
TE Takayuki Enomoto
AE Atena Endo
SO Seiji Okada
KA Kimi Araki
NS Nobuaki Shiraki
SK Shoen Kume
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Freshly isolated mice islets were introduced to the atelocollagen sponge scaffold immediately before the Live/Dead assay. Calcein-AM (green, live) and ethidium homodimer (red, dead) were used to stain the islet-carried scaffold and naked islets according to the manufacturer’s protocol (R37601, ThermoFisher Scientific). Images were acquired with an Olympus BX51-FL (Olympus, Japan). Quantification of the cell viability was carried out by calculating the area of green (live) and red (dead) fluorescence using ImageJ. % cell viability: the green area versus the total islet area.

Copyright and License information: The Author(s) ©2024
This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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