Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
COLO205 or HCT116 colorectal cancer cells (1 × 104 cells/well) were seeded onto an opaque white 96-well plate 1 day before treatment. COLO205 or HCT116 cells were treated with SP2509 or doxorubicin for 9 and 24 hours, respectively. Post-treatment, 100 μL of Caspase-Glo 3/7 reagent (Promega) was added to each of the 100 μL of medium-containing cells. The plate was mixed for 1 minute and then incubated at room temperature for 30 minutes before measurement with a luminometer (USA).
Copyright and License information: ©2024 The Authors; Published by the American Association for Cancer Research
This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
