Metal chelating experiment

The experiment for determining the metal chelating capacity was carried out according to Dinis et al. (Dinis et al., 1994; Hassan et al., 2021) The plant extracts were dissolved in their solvent, and the positive control EDTA was dissolved in methanol at 1 mg/mL 3.2 mL of distilled water was added to the extract solutions, and firstly, 100 µL of FeCl₂ and then 200 µL of ferrozine were added. It was mixed well and incubated for 10 min at room temperature. Afterward, measurements were made at 562 nm in the spectrophotometer. The extracted data’s percent antioxidant activity values were calculated by substituting them in the equation.