Chymotrypsin-like proteasome activity assay

MM Marina Moya-Molina
BD Birthe Dorgau
EF Emily Flood
SL Stef J F Letteboer
EL Esben Lorentzen
JC Jonathan Coxhead
GS Graham Smith
RR Ronald Roepman
SG Sushma Nagaraja Grellscheid
LA Lyle Armstrong
ML Majlinda Lako
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The chymotrypsin-like protease activity was quantified in the ROs after 4 weeks-several doses of PMO treatment (n = 20 ROs per treated group per biological replicate) using the Proteasome Activity Assay Kit (Abcam) following manufacturer’s protocol. Protease activity was measured by the fluorescence intensity (Excitation/Emission = 350/440 nm) using a Varioskan LUX Multimode Microplate Reader and normalized to the protein amount.

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