Luminescent cell viability assay

CellTiter-Glo assay was performed according to manufacturer’s instructions (Promega G7570). Briefly, after counting on day 6, BMDMs were plated at 1x10⁵ cells/well in a 96-well plate in complete RPMI medium and allowed to attach overnight. Next day, the cells were treated with the indicated concentrations of CDT proteins or left untreated and incubated for 4 hours at 37°C with 5% CO₂. CellTiter-Glo Reagent was then added to the equal volume of cell culture medium present in each well. The contents were mixed for 2 minutes on an orbital shaker to induce cell lysis and allowed to incubate at room temperature for 10 minutes to stabilize luminescent signal. Luminescence was recorded on BioTek Cytation 5 plate reader. Relative luminescence units of treatments were normalized to the untreated control (set at 100%) and reported as percentages relative to that.