4.15. m6A RNA immunoprecipitation (meRIP) assay

The sequence‐based RNA adenosine methylation site predictor (SRAMP) online tool was used to predict m⁶A modifications in IGFBP7. Based on the predicted m6A primer pairs, we designed corresponding primers for meRIP‐PCR/qPCR. A Magna MeRIP m⁶A Kit (cat. no. 17–10,499, Millipore Sigma, MA, USA) was used to verify m⁶A modification in GC cells following the manufacturer's instructions. In brief, anti‐m⁶A antibody‐coated magnetic beads were coimmunoprecipitated with 150 μg of fragmented mRNA according to the protocols of the GenSeqTM m6A‐MeRIP Kit (GenSeq, Beijing, China). The eluent was used for m6A RNA elution and purification. PCR and reverse transcription (RT)‐qPCR assays were then performed on the abundance of eluted RNA samples with gene‐specific primers to analyse the mRNA levels of m⁶A sites.