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Cell viability and proliferation assays

EB Emma K Baker
ST Scott Taylor
AG Ankita Gupte
PS Phillip P Sharp
MW Mannu Walia
NW Nicole C Walsh
AZ Andrew CW Zannettino
AC Alistair M Chalk
CB Christopher J Burns
CW Carl R Walkley
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Cell were seeded 24 h prior to drug treatment at 2500/well in 96 well plates. Compounds, diluted in DMSO, were applied (0.01 nM to 10 μM) for 48–72 h and viability quantified using CellTitre-Glo (Promega). Half maximal inhibitory (IC50) values were calculated using Prism 6.0e software. Proliferation rate was determined by counting cells on 4-5 consecutive days using a BioRad TC10 counter (Hercules, CA, USA).

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