Acute GVHD models

MHC-mismatched transplants were performed using C57BL/6 and WT or β₂-AR^−/− BALB/c mice as donors and hosts. BALB/c mice received 792 cGy and C57BL/6 mice 965 cGy irradiation from a [137Cs] source 24 hours prior to transplant. Unless otherwise indicated, BALB/c mice received 7×10⁶ total BM IV from C57BL/6 mice. MHC-matched transplants were performed using 129/SvJ as donor and C57BL/6 as hosts. C57BL/6 mice received 965 cGy from a [137Cs] source 24 h prior to transplant. Male C57BL/6 mice were then IV injected with 2×10⁶ female 129/SvJ bone marrow plus 7.5×10⁶ splenocytes and weight loss was tracked. For experiments with T cell depleted bone marrow (TCD-BM), anti-CD90.2 microbeads were used (Miltenyi Biotec). Syngeneic transplants were performed using C57BL/6. C57BL/6 mice received 965 cGy from a [137Cs] source 24 hours prior to transplant, and were given 7×10⁶ total BM IV from C57BL/6 mice. Following transplant, mice were monitored regularly for weight loss and survival. Mice were considered moribund once weight loss reached 20% of their initial weight. For alloHCT involving purified T cells, 3×10⁶ TCD-BM with or without 0.5×10⁶ PanT splenic T cells sorted from C57BL/6 mice were injected into BALB/c hosts. Conversely, C57BL/6 hosts received 4×10⁶ WT BALB/c TCD-BM with or without 3×10⁶ WT or β-AR^−/− PanT cells.