The antimicrobial activity of human β-defensin 2 (hBD-2) and human neutrophil peptide 1 (hNP-1) (Peptide Institute, Japan) was assayed by the radial diffusion method, as previously described, in pH-defined agar (pH 7.5) [26]. At least 3 biological repeats were performed for each strain/peptide combination.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.