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iPSC colonies from 2 confluent 100 mm dishes were harvested after treatment with StemPro® Accutase® (Life Techonologies), pelleted, and suspended in 300 μL Matrigel. The cells were injected subcutaneously into nude mice; 6 to 8 weeks after injection, teratomas were dissected, fixed overnight in 10% buffered formalin phosphate, and embedded in paraffin. The sections were stained with hematoxylin and eosin for further analysis. The protocols were approved by the University of California San Diego Institutional Animal Care and Use Committee.
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