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KT K A Thomas
NV N M Valenzuela
DG D Gjertson
AM A Mulder
MF M C Fishbein
GP G C Parry
SP S Panicker
ER E F Reed
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Monoclonal mouse anti-human C1s, or TNT003, was made as previously described (37). TNT003, nonspecific isotype control antibody (Control, IgG2a, #BE0085; BioXCell, West Lebanon, NH), anti-C5 (#A217; Quidel, San Diego CA), and a nonspecific isotype control antibody (Control, IgG1, #BE0083; BioXCell) were cleaved to produce F(ab′)2 fragments (F(ab′)2 Prep Kit, #44988; Thermo Scientific, Rockford, IL), thereby removing any nonspecific Fc-mediated effects of the inhibitor. Control F(ab′)2 antibodies were used in assays at the same concentration as the highest dose of TNT003. Single donor normal human serum (NHS) was used as source of active complement (#IPLA-CSERS; Innovative Research, Novi, MI).

Copyright and License information:  ©2015 The Authors. American Journal of Transplantation Published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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