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Sequential evaluation of intranuclear Ikaros in immune cells

KS Kartik Sehgal
RD Rituparna Das
LZ Lin Zhang
RV Rakesh Verma
YD Yanhong Deng
MK Mehmet Kocoglu
JV Juan Vasquez
SK Srinivas Koduru
YR Yan Ren
MW Maria Wang
SC Suzana Couto
MB Mike Breider
DH Donna Hansel
SS Stuart Seropian
DC Dennis Cooper
AT Anjan Thakurta
XY Xiaopan Yao
KD Kavita M Dhodapkar
MD Madhav V Dhodapkar
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To detect changes in intranuclear Ikaros in immune cells following Pom therapy, cells labeled with T and NK cell markers were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set using the recommended protocol. A fluorochrome-conjugated monoclonal antibody against IKZF1 (R32-1149) was used to detect intranuclear levels of the protein.

Copyright and License information:  ©2015 by The American Society of Hematology

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