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Quantification of Infected Cells with LIVE/DEAD Bacterial Viability Kit

EH Erika Hertzén
LJ Linda Johansson
RK Rita Kansal
AH Alexander Hecht
SD Samira Dahesh
MJ Marton Janos
VN Victor Nizet
MK Malak Kotb
AN Anna Norrby-Teglund
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1×105 macrophages were seeded/well on cover slips in a 24-well plate. Cells were infected with S. pyogenes with a MOI of 10 CFU/cell for 2 hours after which antibiotics was added as described above. To detect intracellular bacteria, cells were incubated with LIVE/DEAD Backlight Bacterial viability kit (Invitrogen, Molecular Probes), which enables discrimination between viable and dead bacteria, in accordance with provided protocol. The number of cells harbouring > 2 viable bacteria was enumerated by microscopy analysis.

Copyright and License information:  ©2012-04-12 This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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