Media, serum, and non-cytokine reagents

For moDC cultures, complete RPMI 1640 was supplemented with 10 mM HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid), 1% penicillin/streptomycin (Media Laboratory, MSKCC), 50 μM 2-mercaptoethanol (GibcoBRL Life Technologies), 2 mM L-glutamine (GibcoBRL), and heat-inactivated, autologous single-donor or pooled human serum (1% or 10% vol/vol). For LC cultures, X-VIVO 15 (BioWhittaker) was only supplemented with cytokines (see below). All media and reagents were endotoxin-free.