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16S quantitation was performed using real-time PCR of 20 uL triplicate reactions containing 10 ng stool DNA, TaqMan Environmental Master Mix 2.0 (Applied Biosystems), and primers/probe specific for bacterial 16S rDNA as described by Hill, et al. [10]. Standard curves with a range of 101 to 2×107 copies of E. coli 16S rDNA were prepared using linearized plasmid containing a single copy of the 16S gene.
Copyright and License information: ©2012 Kurzweil et al.
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