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Traf2-TKO mice and age- and sex-matched wild-type control mice (6–8 weeks old) were infected with a low dose (1 × 104 cfu/mouse) of LM-OVA on day 0. After 40 days, mice were injected s.c. with 8 × 105 B16-OVA murine melanoma cells and monitored for tumor growth and survival. Mice with tumor volumes measuring 225 mm2 were considered moribund and sacrificed according to protocols approved by the Institutional Animal Care and Use Committee of the University of Texas MD Anderson Cancer Center.
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