EXPERIMENTAL MODEL AND SUBJECT DETAILS

Itgb6^−/− and Itgb8^loxP mice were kindly provided by Dean Sheppard (University of California, San Francisco). E8I-creER^T2 and ROSA26.LSL.hNGFR reporter mice were developed by Dario A.A. Vignali (University of Pittsburgh) (Hirai et al., 2019). Tgfb^loxP and TGFβRICA mice have been previously described (Bartholin et al., 2008; Liu et al., 2020; Marie et al., 2006). C57BL/6 (WT), B6.SJL-Ptprca Pepcb/BoyJ (CD45.1), Tg(KRT14-cre)1Amc/J (Krt14-cre), C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I), B6.129S7-Rag1tm1Mom/J (Rag^−/−), CBy.PL(B6)-Thy1^a/ScrJ (Thy1.1) mice were purchased from Jackson Laboratories. We crossed Krt14-cre mice with Itgb8^loxP and Itgb6^−/− mice to obtain Itgb6^−/−Itgb8^ΔKC mice (Mohammed et al., 2016). E8I-creER^T2 mice were crossed with TGFβRI-CA mice to obtain TGFβRICA^CD8 mice. TGFβRI-CA^CD8 mice were further crossed with Itgb6^−/− mice to generate Itgb6^−/− x TGFβRI-CA^CD8 mice. E8I-creER^T2 mice were crossed with ROSA26.LSL.hNGFR reporter mice and Tgfb^loxP mice to obtain Tgfb^ΔCD8 mice. Krt14-creER^T2 mice were bred with Tgfb^loxP mice and ROSA26.LSL.YFP (Jackson Laboratories) reporter mice resulting Tgfb^ΔKC mice. We generated Thy1.1⁺Rag^−/−OT-I mice by crossing OT-I mice with Rag^−/− and Thy1.1 mice. TGFβRICA^CD8 mice and Thy1.1⁺Rag^−/−OT-I mice were bred to generate OT-I TGFβRI-CA mice. OT-I cells carrying ROSA26-creER^T2 and floxed TGFβ receptor II gene (OT-I creER^T2 TGFbRII^fl/fl) were kindly provided by Thorsten Mempel (Harvard University). We used age- and sex-matched (males and female) mice that were between 6 and 12 weeks of age in all experiments. All mice were maintained under specific-pathogen-free conditions and all animal experiments were approved by University of Pittsburgh Institutional Animal Care and Use Committee.