PA imaging was performed at 24 h post-injection using the Vevo 3100 Imaging System (FUJIFILM VisualSonics, Canada) as described before [33]. The system was equipped with Vevo LAZR-X cart, a Vevo LAZRTight Enclosure, and a Vevo Imaging Station. Mice were anesthetized and placed on a preheated imaging table. The MX550D transducer was used for US and PA imaging (FUJIFILM, VisualSonics; 25–55 MHz; axial resolution: 40 µm; excitation 780 nm). Images were analyzed using the Vevo LAB (version: 5.5.0, FUJIFILM, VisualSonics). Tumor-to-background ratios (TBRs) were calculated using the following formula: TBR = PAtumor/PAbackground.
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