In vivo PA imaging

RH Ruben D Houvast
NB Nada Badr
TM Taryn March
LM Lysanne D A N de Muynck
VS Vincent Q Sier
TS Timo Schomann
SB Shadhvi Bhairosingh
VB Victor M Baart
JP Judith A H M Peeters
GW Gerard J P van Westen
AP Andreas Plückthun
JB Jacobus Burggraaf
PK Peter J K Kuppen
AV Alexander L Vahrmeijer
CS Cornelis F M Sier
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PA imaging was performed at 24 h post-injection using the Vevo 3100 Imaging System (FUJIFILM VisualSonics, Canada) as described before [33]. The system was equipped with Vevo LAZR-X cart, a Vevo LAZRTight Enclosure, and a Vevo Imaging Station. Mice were anesthetized and placed on a preheated imaging table. The MX550D transducer was used for US and PA imaging (FUJIFILM, VisualSonics; 25–55 MHz; axial resolution: 40 µm; excitation 780 nm). Images were analyzed using the Vevo LAB (version: 5.5.0, FUJIFILM, VisualSonics). Tumor-to-background ratios (TBRs) were calculated using the following formula: TBR = PAtumor/PAbackground.

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