2.6. The colony stimulating factor 1 receptor (CSF1R) antagonist, PLX5622 administration

PLX5622 was provided by Plexxikon Inc. (Berkley, CA) and formulated in AIN‐76A rodent chow by Research Diets Inc. (New Brunswick, NJ) at a concentration of 1,200 ppm (Spangenberg et al., 2016). According to the provider, the specialized diet was stored in a 4°C refrigerator. Mice were provided ad libitum access to PLX5622 diet or AIN‐76A chow as vehicle control. After 2 weeks on PLX5622 or vehicle chow, mice were subjected to CCI injury and examined for acidosis and inflammation by flow cytometry and qRT‐PCR.