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Cells were briefly washed with PBS and incubated with 1 μg/ml acridine orange hydrochloride solution (Invitrogen) in PBS for 20 min at RT. Then, the cells were analyzed using confocal microscopy and flow cytometry. The acidic autophagic vacuoles emitted bright red fluorescence, while green fluorescence was observed in the cytoplasm and nucleus.
Copyright and License information: Copyright ©2020 Lee, Lee, Chen, Lee, Lai, Lin, Hsu, Sung, Lee and Chen
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