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DNFB induced Contact Hypersensitivity.
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Mice were sensitized with 50 μl 0.5% DNFB (1-fluoro-2,4-dinitrobenzene; Millipore Sigma) in acetone:olive oil (4:1) on shaved abdomens then challenged 5 days later with 25 μl 0.5% DNFB in acetone:olive oil (4:1) on the right ear and 25 μl acetone:olive oil (4:1) (vehicle) on the left ear. Ear thickness was measured with a micrometer before challenge and after 24 hours.
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