Cell lines

Use of human SCLC cell lines NCI-H69, H69M (parental, control, and STING KO) NCI-H82, NCI-H2081, and NCI-H196 was continued from our prior work⁶. The human SCLC cell line, CORL47, was kind provided by Dr. M. Oser. The murine lymphoma cell line, BW5147.3 was obtained from the laboratory of E. Reinherz. COLO668 and 293T cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD).

H69, H69M, H69EZ-G, H82, H2081, and CORL47 were cultured in RPMI-1640 (Thermo Fisher Scientific, #11875–119) containing 10% FBS (Cat# 100–106, Gemini Bio-products, West Sacramento, CA), 1X penicillin/streptomycin (Cat# 15140122, Thermo Fisher Scientific, Waltham, MA), and prophylactic Plasmocin (2.5 mg/mL; Cat# ant-mpp, Invivogen, San Diego, CA). H196 and 293T were maintained in Dulbecco’s Modified Eagles Medium (DMEM) (Cat# 11965–118, Thermo Fisher Scientific) containing 10% FBS and 1X penicillin/streptomycin. RPP and RPP-A cell lines were cultured in RPMI (Cat# 11875–119, Thermo Fisher Scientific), containing 10% FBS (Cat# 100–106, Gemini Bio-products), 1X penicillin/streptomycin (Cat# 400–109, Gemini Bio-Products, Sacramento, CA), and HITES [1X insulin-transferrin-selenium (Cat# I3146, Sigma-Aldrich, St. Louis, MO), 10 nM β-estradiol (Cat# E2257, Sigma-Aldrich), and 10 nM hydrocortisone (Cat# 10687010, Thermo Fisher Scientific)]. BW5147.3 was maintained in DMEM (Cat# 11965–118) containing 10% FBS, 1X penicillin/streptomycin (Cat# 15070063), 400 μg/mL G418 (Cat# 10131035) (Thermo Fisher Scientific), and 400 μg/mL hygromycin (Cat# H0135, Sigma-Aldrich). All cell lines were routinely monitored for mycoplasma using the Takara PCR Mycoplasma Detection Set (Cat# 6601, Kusatsu, Shiga, Japan), and were negative.