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Methylcellulose colony-formation assay
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To assess the frequency of colony-forming cells (CFCs), the colony-forming unit (CFU) Assay was performed by FACS sorting single, live (7AAD-) CD34highEGFPhigh and/or CD45- cells into methylcellulose (MethoCult H4230, STEMCELL Technologies) supplemented with SCF (25 ng/ml), GM-CSF (50 ng/ml), IL-3 (25 ng/ml) (Peprotech) and EPO (5U/ml, Apoteket) 3 days post-electroporation (7 days in culture). Plates were incubated at 37 °C, 5% CO2 and mature hematopoietic colonies were assessed after 13 days.
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