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To assess cytokine release by CAR-T cells, supernatants were harvested 12 hours after incubation with different types of tumor cells for assessment with a Cytometric Bead Array (CBA, Biolegend, Cat#: B314528) kit following the manufacturer’s protocol. Beads were analyzed by a flow cytometry assay. Each experiment was done in triplicate.
Copyright and License information: Copyright ©2021 Li, Feng, Shang, Yu, Wang, Zhang, Song, Wang, Shi and Wang
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