scRNA‐Seq data and ImmGen gene expression analysis

Single-cell sequencing data of thymic iNKT cells were downloaded from published scRNA-seq data (GSE152786). Data analysis was performed using functions available in the Seurat (v3.1.4) package of R as described by Krovi et al. [9].

Relative expression of Srsf1 and selected genes in T and iNKT cell populations (Fig. 1A) were analyzed by publicly available data from the Immunological Genome Project (ImmGen) gene expression database (GSE15907).

SRSF1 is dynamically expressed in iNKT cell subsets and potentially binds multiple iNKT-related genes. A Analysis of Srsf1 expression in various T cell subsets by published microarray data (GSE15907). B The expression level of Srsf1 in thymic iNKT cells is illustrated in dot plots by published single-cell RNA-seq data (GSE152786). Left: Uniform manifold approximation and projection (UMAP) of iNKT cells was colored by inferred cluster identity; middle: The expression of Srsf1 is plotted along a colorimetric gradient, with red corresponding to high expression; right: Violin plots showing the aggregate expression level of Srsf1 from Cluster 0 to Cluster 10. C Overlapping SRSF1-binding genes in thymocytes (GSE141349) and iNKT-related genes. Sixty overlapping genes were identified, and iNKT-associated regulators in each category are shown