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Cholesterol quantification with Amplex™ red cholesterol assay kit
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Equimolar concentrations (300 µM) of cholesterol and linoleic acid were added to 0.1 M sodium phosphate buffer (pH 6). Free cholesterol was then quantified before (timepoint zero) or after the addition of recombinant Lip2 to samples and incubation at 37 °C with shaking. To that end, Amplex™ red cholesterol assay kit was used according to the manufacturer’s instructions.
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