2.2. NAB2::STAT6 fusion assessment

NAB2::STAT6 fusion status was successfully assessed in n = 101 cases, as previously described [1]. In brief, RNA extraction was performed using the Qiagen miRNeasy FFPE kit by methods previously described by Wang et al. [20]. Screening of the most common NAB2::STAT6 fusions was performed, including exon 4::exon 2, exon 4::exon 3, exon 6::exon 16, and exon 6::exon 17 using single‐plex PCR with subsequent agarose gel electrophoresis, identifying NAB2::STAT6 gene fusions. Samples with no results by single‐plex PCRs were analyzed by next‐generation sequencing using the Archer FusionPlex Sarcoma Kit (Archer Dx Inc, Boulder, CO) to detect fusions of 26 genes employing the Anchored Multiplex PCR‐based enrichment.