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2.15. Nrf2 transcription factor assay
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NRK52E cells were cultured in 10-cm dishes until 70% confluent and incubated for MG 500 μM at 30 min, and 1h. The medium was aspirated off. Cells were rinsed once with PBS. Nuclear extracts were harvested and suspended in 300 μL of an analytical solution, and Nrf2 transcription factor activity was measured using a Nrf2 Transcription Factor Assay Kit (Abcam, Boston, MA, USA). The activity was measured at OD 450 nm.
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