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2.4. Bioinformatics Analyses

QX Qi Xiao
MQ Meng Qu
JX Jianping Xie
CZ Cigen Zhu
YS Yuping Shan
AM Aihua Mao
WQ Wenxian Qian
JZ Jiaping Zhu
JG Jiahui Guo
DL Dong Lang
JN Jiaqiang Niu
LW Libin Wen
KH Kongwang He
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The complete genomes of the following eight reference PCV2 strains were downloaded from GenBank: PCV2a (AF381175), PCV2b (AY181945), PCV2c (EU148503), PCV2d (AY181946), PCV2e (KT795289), PCV2f (MF278779), PCV2g (JX099786), and PCV2h (JX506730) [16,34]. The Lasergene DNAStar software (7.1) was used to analyze the nucleotide sequences (complete, ORF1, and ORF2) and their corresponding amino acid (aa) sequences (Cap and Rep) of 251 novel PCV2 strains and reference strains. Phylogenetic trees were generated based on the complete genome sequences using the neighbor-joining method with maximum composite likelihood model in MEGA 7.0 software with 1000 bootstrap replications.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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