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Histopathology evaluation

KS Kátia Nunes da Silva
FM Fernanda Martins Marim
GR Gisele Vieira Rocha
ZC Zaquer Suzana Munhoz Costa-Ferro
LF Luciana Souza de Aragão França
CN Carolina Kymie Vasques Nonaka
BP Bruno Diaz Paredes
ER Erik Aranha Rossi
EL Erick Correia Loiola
CA Corynne Stephanie Ahouefa Adanho
RC Rachel Santana Cunha
MS Mayck Medeiros Amaral da Silva
FC Fernanda Ferreira Cruz
VC Vivian Vasconcelos Costa
DZ Dalila Lucíola Zanette
CR Clarissa Araújo Gurgel Rocha
RA Renato Santana Aguiar
PR Patricia Rieken Macedo Rocco
BS Bruno Solano de Freitas Souza
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The left lung was fixed (4% buffered formalin) and paraffin-embedded for microtomy into 4 μm-thick sections stained with hematoxylin and eosin. They were completely scanned to obtain a panoramic view of the lung parenchyma (3DHISTECH, Budapest, Hungary). Photomicrographs at × 25, × 100, and × 400 magnification were obtained from 10 non-overlapping fields of view per section in the digitalized images. Diffuse alveolar damage (DAD) was quantified using a weighted scoring system by two investigators (F.F.C., P.R.M.R) blinded to group allocation. Six features: hemorrhage, alveolar collapse, inflammation, and edema were graded on scales of 0 to 4 in terms of severity (0, no effect; 4, maximum severity) and extent (0, not visualized; 4, (complete involvement). Final scores, ranging from 0 to 16, were then calculated as the product of the severity and extent of each feature. The cumulative DAD score thus ranged from 0 to 64 [26, 27].

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