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Alkaline phosphatase staining was performed following the protocol of Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich). Cells were fixed in Citrate-Acetone-Formaldehyde solution for 30 s and gently washed in deionized water for 45 s, followed by stained in diluted Naphthol AS-BI Alkaline Solution at room temperature for 15 min, and visualized under bright-field microscopy.
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