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For colony assays with mouse cells, indicated numbers of Lin− bcCML cells or cKit+ cells were plated in methylcellulose medium (M3234, StemCell Technologies). Colonies were scored at 7d. Colony assays with human cell lines or patient derived AML samples, cells were plated in complete methylcellulose medium (H4434 StemCell Technologies). Colony numbers were counted 10–14 days after plating. Taurine antagonist or TAG (synthesized by Enamine Inc.; 92% pure), mTOR activator (MHY1485; Sigma-Aldrich) and Venetoclax (ABT-199; Tocris Bioscience) were used as described.
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