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Cells were transfected with Lipofectamine 2000 (#11668019 Invitrogen) according to the manufacturer’s instructions. We used pcDNA AXL construct for overexpression donated by R. M. Melillo [#105932, Addgene (52)] and AXL siRNA (#AM16708 Id1313 and Id1218, Invitrogen) or scramble siRNA (#AM4635, Invitrogen). Knockdown and overexpression efficiency was assessed at 72 hours after transfection by RT-qPCR or Western blotting as previously described.
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