The preparation of amine-terminated, generation 5 polyamidoamine (G5-PAMAM, hereafter called G5) dendrimer-siRNA nanoparticles for use in mouse models was conducted according to the protocol established by Prof. Daoguang Yan from Jinan University. Briefly, in vivo siRNA delivery was conducted using G5 (CYD-150 A), obtained from Weihai Chenyuan Molecular New Materials Co., Ltd. The N: P ratio of G5 to siRNA was set at 30:1, and the complexes were formed in PBS at room temperature with gentle vortexing for 10 min. A dosage of 1.0 mg/kg of the G5-siRNA complex was administered via tail vein injection every 3 days.
Copyright and License information: The Author(s) ©2024 Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.
Request a Protocol
Ask a
question
Post a Question
