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E.G7 tumors were digested with 1 mg/mL collagenase D supplemented with 10 U/mL DNase I for 30 min at 37 °C prior to centrifugation on a discontinuous Percoll gradient (GE Healthcare). The maximal tumor burden permitted by the ethics committee is no more than 2000 mm3. Tumors were harvested for imaging and tumor weight were measured.
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