Cytospin and May–Grünwald–Giemsa staining

Cytospins were prepared on coated slides with 1 × 10⁵ cells using the Thermo Fisher Scientific Shandon Cytospin 4 centrifuge. The slides were then stained with May–Grünwald–Giemsa solution (Catalog No. MG500, Sigma Aldrich) for 5 min. After rinsing for 90 s in 40 mM Tris buffer at pH 7.4, the slides were stained with Giemsa solution (Catalog No. GS500, Sigma-Aldrich) for 15 min. Finally, the slides were rinsed twice with water. The cells were imaged using a Leica DM2000 inverted microscope.