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Apoptosis induced by staurosporine
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Mobilized CD34+ hematopoietic stem cells were maintained in StemSpan SFEM medium (Stemcell Technologies, Tukwila, WA) with 50ng/mL SCF, 50ng/mL TPO and 50ng/mL Flt-3 ligand. 2uM staurosporine was directly added into the culture medium and the cells treated for 4 hours. After treatment, cells were washed with SFEM medium twice and re-cultured in SFEM medium with 50ng/mL SCF, 50ng/mL TPO and 50ng/mL Flt-3 ligand for 24 hours for Annexin V staining and flow cytometry assays.
Copyright and License information: ©2015 Yan et al
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
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