4.3. Evaluation of compounds in GroEL/ES and HSP60/10-mediated dMDH and dRho refolding assays.

Anne-Marie Ray; Nilshad Salim; Mckayla Stevens; Siddhi Chitre; Sanofar Abdeen; Alex Washburn; Jared Sivinski; Heather M O’Hagan; Eli Chapman; Steven M Johnson

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4.3. Evaluation of compounds in GroEL/ES and HSP60/10-mediated dMDH and dRho refolding assays.

AR Anne-Marie Ray

NS Nilshad Salim

MS Mckayla Stevens

SC Siddhi Chitre

SA Sanofar Abdeen

AW Alex Washburn

JS Jared Sivinski

HO Heather M O’Hagan

EC Eli Chapman

SJ Steven M Johnson

This method is extracted from research article: Bioorg Med Chem, Apr 2021

Exploiting the HSP60/10 Chaperonin System as a Chemotherapeutic Target for Colorectal Cancer

DOI: 10.1016/j.bmc.2021.116129

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All compounds were evaluated for inhibiting E. coli GroEL/ES and human HSP60/10-mediated refolding of the denatured MDH and denatured Rho reporter enzymes as per previously reported procedures.^{84–87,91,92} Detailed protocols for these assays are presented in the Supporting Information.

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