5.3. Evaluation of compounds in GroEL/ES and HSP60/10-mediated dMDH and dRho refolding assays.

Siddhi Chitre; Anne-Marie Ray; Mckayla Stevens; Emma H Doud; Hope Liechty; Alex Washburn; Katelyn Tepper; Jared Sivinski; Heather M O’Hagan; Millie M Georgiadis; Eli Chapman; Steven M Johnson

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5.3. Evaluation of compounds in GroEL/ES and HSP60/10-mediated dMDH and dRho refolding assays.

SC Siddhi Chitre

AR Anne-Marie Ray

MS Mckayla Stevens

ED Emma H Doud

HL Hope Liechty

AW Alex Washburn

KT Katelyn Tepper

JS Jared Sivinski

HO Heather M O’Hagan

MG Millie M Georgiadis

EC Eli Chapman

SJ Steven M Johnson

This method is extracted from research article: Bioorg Med Chem, Oct 2022

Bis-aryl-α,β-unsaturated ketone (ABK) chaperonin inhibitors exhibit selective cytotoxicity to colorectal cancer cells that correlates with levels of aberrant HSP60 in the cytosol

DOI: 10.1016/j.bmc.2022.117072

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All compounds were evaluated for inhibiting E. coli GroEL/ES and human HSP60/10-mediated refolding of the denatured MDH and denatured Rho reporter enzymes as per previously reported procedures.^{29, 55–60, 71, 72} Detailed protocols for these assays are presented in the Supporting Information.

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