2.6. Tumor cell colony formation assay

LAPC-4-AI and PC-3 cells were treated with vehicle control, 40μM EGCG+5μM Q, 5nM Doc or EGCG+Q+Doc for 48h. A 24-well plate was coated with 200μl Matrigel per well and incubated in 37°C for 20 min. After trypsinization 2×10⁴ cells in 200μl complete medium were added to each well. Cells were incubated for 5 days. 200μl of fresh medium was added every two days. Pictures were taken from three fields of each well under a microscope at ×100 magnification. The nmber of cell colony which contained at least 10 cells were counted. The experiment was perfored in triplicate.