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THP1-Lucia™ ISG cells express the secreted luciferase (Lucia) reporter gene under the control of five interferon-stimulated response elements (ISRE). This ISRE luciferase activity of Lucia ISG cells represents the activation of STING signaling. Cells were stimulated with cGAMP, native EVs, Ctrl EVs, or increasing concentrations of cGAS EVs for 13 h. The ISRE reporter activity was determined following the standard protocol (Invitrogen).
Copyright and License information: ©2024 The Author(s) 2024. Published by Oxford University Press on behalf of Higher Education Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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