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Analysis of immune cells infiltration in the tumor microenvironment by flow cytometry
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Immune cells including Tregs, myeloid-derived suppressor cells (MDSCs) and lymphocytes that had infiltrated into the tumor microenvironment were analyzed by flow cytometry. Briefly, tumor tissues were digested with 2 U/mL dispase II and 100 μg/mL DNase I in PBS at 37 °C for 30 min to generate single-cell suspension. Cells were stained with antibodies, then analyzed by flow cytometry to identify immune cells.
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