In vitro stimulation of RAW264.7 macrophages with LPS

ZH Zi-Yi Han
ZF Zhuang-Jiong Fu
YW Yu-Zhang Wang
CZ Cheng Zhang
QC Qi-Wen Chen
JA Jia-Xin An
XZ Xian-Zheng Zhang
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RAW264.7 macrophages were cultured in 6-well plates for 24 hours. Subsequently, the cells were treated with 100 ng mL−1 of LPS and incubated for an additional 24, 48, and 72 hours to evaluate the M2 polarized state using FCM.

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