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Cord blood-derived megakaryocytes

TM Thomas Moreau
AE Amanda L. Evans
LV Louella Vasquez
MT Marloes R. Tijssen
YY Ying Yan
MT Matthew W. Trotter
DH Daniel Howard
MC Maria Colzani
MA Meera Arumugam
WW Wing Han Wu
AD Amanda Dalby
RL Riina Lampela
GB Guenaelle Bouet
CH Catherine M. Hobbs
DP Dean C. Pask
HP Holly Payne
TP Tatyana Ponomaryov
AB Alexander Brill
NS Nicole Soranzo
WO Willem H. Ouwehand
RP Roger A. Pedersen
CG Cedric Ghevaert
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Cord blood was obtained after informed consent under a protocol approved by the Cambridgeshire 4 Research Ethics Committee (07/MRE05/44). CD34-positive cells (≥98%) isolated by magnetic cell sorting (Myltenyi Biotec) were seeded at 1E+5 cells per ml in CellgroSCGM containing TPO (100 ng ml−1) and IL1-β (10 ng ml−1) and cultivated for 10 days. We routinely obtained 70–90% CD41a+ and 20–60% CD42a+ cells by the end of the culture.

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