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Eligible patients received four vaccinations of 1×106 to 1×107 TL-pulsed DCs at weekly intervals. Immunization was subsequently continued at monthly intervals for up to 12 vaccinations, depending on clinical response. Before injection, DCs were washed three times in sterile PBS and resuspended in a total volume of 0.5 ml PBS. DC preparations were confirmed to be endotoxin-free (<10 pg/ml in the supernatant). DCs were then administered immediately into an inguinal lymph node under ultrasound control.
Copyright and License information: Springer-Verlag ©2003
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