Normal human bone marrow stromal cells

These cells were delivered in frozen vials (Cambrex) and stored in liquid nitrogen until use in the coculture assay. The cells were derived from a healthy 20-year-old white female. Bone marrow mononuclear cells were then derived by gradient separation (specific density 1.077) and cultured in Myelocult growth medium (BioWhitacker) for 4 weeks. The stromal cells represent the adherent cell population of the cultured cells and are a heterogeneous population of fibroblasts, reticulum cells, endothelial cells, macrophages and fat cells. The cells showed a purity of 95% and tested negative for mycoplasma, human immunodeficiency virus 1, hepatitis B and hepatitis C (polymerase chain reaction) (distributor’s information).