RNA Isolation and Northern Blot Analysis

RNA isolation and Northern blot analysis were performed according to the procedure described previously [14]. Twelve micrograms of total RNAs from each sample was loaded in each lane. The probe was a ³²P-labeled Glb1l4 cDNA fragment (from 2279 to 2615 bp in cDNA; total of 337 bp). An 18s r-RNA hybridization signal was used as a loading control. Autoradiographs with pronounced differences in expression were analyzed by densitometry.